A rapid method to identify cosmids containing rare restriction site
نویسندگان
چکیده
منابع مشابه
Rapid acquisition of unknown DNA sequence adjacent to a known segment by multiplex restriction site PCR.
The determination of unknown DNA sequences around a known locus has important applications in molecular genetics, specifically in genomic walking and genome mapping. Several PCR-based methods have been reported to address this issue, but they often involve multiple, time-consuming steps. We have previously described a technique known as restriction site PCR (RS-PCR) that allows sequence acquisi...
متن کاملA Graph-based Method to Detect Rare Events: An Application to Identify Pathologic Cells
Detection of outliers and anomalous behavior is a well known problem in the field of data mining and statistics. Although the problem of identifying single outliers has been extensively studied in the literature, little or some effort has been consecrated to the detection of small groups of outliers which are similar to each other but markedly different from the entire population. Many real wor...
متن کاملDevelopment of a rapid, one-step-visual method to detect Salmonella based on IC-LAMP method
Background: Salmonella can cause serious human gastroenteritis and is frequently isolated from various food samples. The cell culturing, immunoassay, and polymerase chain reactions (PCR) are the current methods to detect such pathogenic agents. However, these methods are time-consuming and labor-intensive, and thus unavailable for rapid-monitoring of Salmonella</em...
متن کاملsimple and rapid identification of most medically important candida species by a pcr-restriction enzyme method
opportunistic fungal infections including candidiasis have increased dramatically in recent years. most medically important fungi are candida species. rapid identification od candida isolates to the species level in the clinical laboratory is necessary for more rapid and effective antifungal therapy and to facilitate hospital infection control measures. conventional morphological methods for id...
متن کاملUniversal restriction site-free cloning method using chimeric primers.
A universal restriction site-free cloning method has been developed to precisely insert a DNA fragment into a vector at any desired location without altering any nucleotide(s) in either the DNA fragment or the vector. The technique employs two pairs of chimeric primers, each containing a ribonucleotide. One pair of primers is used to amplify a target DNA fragment and another is used to prepare ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1987
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/15.4.1415